Rabbit anti‑Bovine COL1A1 / Collagen I Alpha 1 Polyclonal RW-C744029-0.1

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Antibody: COL1A1 / Collagen I Alpha 1 Rabbit anti-Bovine Polyclonal Antibody, Application: IHC-P, IF, WB, ELISA, RIA, Reactivity: Bovine, Human, Pig, Sheep, Cow, Format: Unconjugated, Unmodified, Descriptio: Collagen I Alpha 1 antibody RW-C744029 is an unconjugated rabbit polyclonal antibody to Collagen I Alpha 1 (COL1A1) from bovine. It is reactive with human, bovine, cow and other species. Validated for ELISA, IF, IHC, RIA and WB., Targe: Bovine COL1A1 / Collagen I Alpha 1, Synonym: COL1A1 | Alpha-1 type I collagen | Collagen alpha 1 chain type I | Collagen alpha-1(I) chain | Collagen, type I, alpha 1 | Collagen I | Pro-alpha-1 collagen type 1 | OI4, Hos: Rabbit, Reactivit: Bovine, Human, Pig, Sheep, Cow (tested or 100% immunogen sequence identity), Clonalit: Polyclonal, Conjugation: Unconjugated, Modification: Unmodified, Immunoge: Purified collagen type I from bovine skin, Application: IHC - Paraffin (1:1000)
Immunofluorescence (1:80)
Western blot
ELISA (1:400)
Radioimmunoassay, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type I Collagen usually exists as a heterotrimer formed by alpha 1(I) and alpha 2(I) chains and is found in bone, cornea, skin and tendon. In foetal tissues also homotrimers of alpha-1(I) are found, but they are not constituents of normal adult tissues. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Bovine collagen type I 100%, bovine collagen II, IX, XI <0.1%, sheep collagen type I 50 % (IHC estimated), bovine fibronectin <0.1%, human collagen type I <2%; chicken collagen type I <0.1% (estimates except sheep collagen I from solid phase RIA at a 1:500 dilution )., Presentatio: Lyophilized from PBS, Reconstitutio: Reconstitute in 100 µl of distilled water., Storag: Store at -20°C., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About COL1A1 / Collagen I Alpha : Uniprot: P02452 NCBI: NM_000088 NP_000079.2

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