Rabbit anti‑Bovine ALPI / Alkaline Phosphatase IgG Polyclonal RW-C209238-100

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Antibody: ALPI / Alkaline Phosphatase Rabbit anti-Bovine Polyclonal (Lyophilized) (HRP) Antibody, Application: IHC, WB, ELISA, Reactivity: Bovine, Format: Horseradish Peroxidase, Lyophilized, Descriptio: Alkaline Phosphatase antibody RW-C209238 is an HRP-conjugated rabbit polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA, IHC and WB., Targe: Bovine ALPI / Alkaline Phosphatase, Synonym: ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP, Hos: Rabbit, Reactivit: Bovine (tested or 100% immunogen sequence identity), Clonalit: IgG Polyclonal, Conjugation: Horseradish Peroxidase, Purificatio: Delipidation, salt fractionation and ion exchange chromatography followed by dialysis., Modification: Lyophilized, Immunoge: Alkaline Phosphatase [Calf intestine]., Specificit: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined., Application: IHC
Western blot (1:200 - 1:1000)
ELISA (1:2000 - 1:10000), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Anti-Alkaline phosphatase has been assayed against 1.0 ug of Alkaline Phosphatase [E. coli] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3 to 1:15000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested., Presentatio: Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide, Reconstitutio: Reconstitute in 100 µL of deionized water., Storag: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee., About ALPI / Alkaline Phosphatas: Uniprot: P09923 NCBI: NM_001631 NP_001622.2

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