Rabbit anti‑Akt Substrate IgG Polyclonal RW-C63885-100

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Antibody: Akt Substrate Rabbit Polyclonal (pSer/Thr) Antibody, Application: IHC, IHC-P, WB, IP, ELISA, Format: Unconjugated, Unmodified, Descriptio: Akt Substrate antibody RW-C63885 is an unconjugated rabbit polyclonal antibody to Akt Substrate (pSer/Thr). Validated for ELISA, IHC, IP and WB., Targe: Akt Substrate, Hos: Rabbit, Clonalit: IgG Polyclonal, Conjugation: Unconjugated, Purificatio: Immunoaffinity purified, Modification: Unmodified, Immunoge: Synthetic phospho- Akt substrate peptides., Epitop: pSer/Thr, Specificit: Preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA the antibody recognizes a wide range of phosphorylated Akt substrate peptides and by 2D gel Western blot analysis it recognizes a large number of presumptive Akt substrate proteins., Application: IHC
IHC - Paraffin
Western blot (1:1000)
Immunoprecipitation (1:20)
ELISA (1:1000), Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Western Blot: 1:1000. Immunoprecipitation 1:20. Immunohistochemistry (Paraffin): 1:100. ELISA: 1:1000., Presentatio: 10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 50% Glycerol, 0.1mg/ml BSA, Storag: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles., Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.

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